- Title
- Immunization with a heat-killed preparation of the environmental bacterium Mycobacterium vaccae promotes stress resilience in mice
- Creator
- Reber, Stefan O.; Siebler, Philip H.; Greenwood, Benjamin N.; Jansch, Charlie; Lechner, Anja; Schmidt, Dominic; Uschold-Schmidt, Nicole; Füchsl, Andrea M.; Langgartner, Dominik; Walker, Frederick R.; Hale, Matthew W.; Perez, Gerardo Lopez; Donner, Nina C.; Morton, James T.; Smith, David G.; Kopelman, Jared M.; Lowe, Kenneth R.; Wheeler, Kristen J.; Fox, James H.; Hassell Jr., James E.
- Relation
- Proceedings of the National Academy of Sciences Vol. 113, Issue 22, p. E3130-E3139
- Publisher Link
- http://dx.doi.org/10.1073/pnas.1600324113
- Publisher
- National Academy of Sciences
- Resource Type
- journal article
- Date
- 2016
- Description
- The prevalence of inflammatory diseases is increasing in modern urban societies. Inflammation increases risk of stress-related pathology; consequently, immunoregulatory or antiinflammatory approaches may protect against negative stress-related outcomes. We show that stress disrupts the homeostatic relationship between the microbiota and the host, resulting in exaggerated inflammation. Repeated immunization with a heat-killed preparation of Mycobacterium vaccae, an immunoregulatory environmental microorganism, reduced subordinate, flight, and avoiding behavioral responses to a dominant aggressor in a murine model of chronic psychosocial stress when tested 1-2wk following the final immunization. Furthermore, immunization with M. vaccae prevented stress-induced spontaneous colitis and, in stressed mice, induced anxiolytic or fear-reducing effects as measured on the elevated plus-maze, despite stress-induced gut microbiota changes characteristic of gut infection and colitis. Immunization with M. vaccae also prevented stress-induced aggravation of colitis in a model of inflammatory bowel disease. Depletion of regulatory T cells negated protective effects of immunization with M. vaccae on stress-induced colitis and anxiety-like or fear behaviors. These data provide a framework for developing microbiome- and immunoregulation-based strategies for prevention of stress-related pathologies.
- Subject
- anxiety; chronic psychosocial stress; fear; microbiota; posttraumatic stress disorder
- Identifier
- http://hdl.handle.net/1959.13/1328315
- Identifier
- uon:25874
- Identifier
- ISSN:0027-8424
- Rights
- Freely available online through the PNAS open access option.
- Language
- eng
- Full Text
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